Journal: Cancer Cell International

Article Title: PIBF1 (p.R405Q) germline variant identified in cancer susceptibility family impairs protein stability and function

doi: 10.1186/s12935-025-04132-y

Figure Lengend Snippet: PIBF1 (p.R405Q) mutation is a candidate gene for hereditary cancer syndromes. A . The expression of PIBF1 is low in BRCA patients in the UALCAN database. B . The expression level of PIBF1 in the UALCAN database is negatively correlated with the tumor grade. C . The Kaplan-Meier Plotter plots survival curves of PIBF1 and low expression of PIBF1 was correlated with poor prognosis of breast cancer. D , E . Q-PCR and WB detection of PIBF1-WT and PIBF1(p.R405Q) in the MDA-MB-231 and MDA-MB-468. F . CCK8 was performed to detect the effect of PIBF1-WT and PIBF1(p.R405Q) on the cell growth of breast cancer cells. G , H . Plate cloning assay to detect the effect of PIBF1-WT and PIBF1(p.R405Q) on the proliferation of breast cancer cells. I . Transwell assay to detect the effect of PIBF1-WT and PIBF1(p.R405Q) on the invasion ability of breast cancer cells. J . WB assay to detect the regulatory effect of PIBF1-WT and PIBF1(p.R405Q) on ERK phosphorylation. All data are presented as mean ± SEM ( n = 3 independent cell culture experiments), *, P < 0.05; **, P < 0.01; ***, P < 0.001

Article Snippet: The medium was refreshed every 24 h with a 1:10 dilution of CCK8 reagent (Cat#C0005, TargetMol, USA) and incubated at 37 °C for 3 hours before measuring absorbance at 450 nm using a microplate reader (EnSpire 2300, PerkinElmer, USA).

Techniques: Mutagenesis, Expressing, Cloning, Transwell Assay, Phospho-proteomics, Cell Culture